Session Index

S6. Biophotonics and Biomedical Imaging

Biophotonics and Biomedical Imaging II
Friday, Dec. 1, 2023  15:15-17:30
Presider: Prof. Guan-Yu Zhou (China Medical University, Taiwan)
Room: 92271 (2F)
15:15 - 15:45
Manuscript ID.  1088
Paper No.  2023-FRI-S0602-I001
Invited Speaker:
Hsiang-Chieh Lee
Development of high-speed optical coherence tomography technology for biomedical imaging and industry applications
Hsiang-Chieh Lee, National Taiwan University (Taiwan)

Optical coherence tomography (OCT) is a non-invasive optical imaging technology that can provide real-time and two- or three-dimensional information about the sample architecture based on intrinsic optical scattering contrast. The emergence of the second generation of OCT – Fourier-domain (OCT) with so-called Fourier-domain interferometry has revolutionized OCT in biomedical applications, leveraging the increased imaging speed, improved detection sensitivity, and extended imaging range, which also leads to adding the functional information of the imaging tissue, for example, OCT angiography (OCTA) and polarization-sensitive OCT (PS-OCT). In addition, the FD-OCT also further reduced the system complexity, beneficial to the broader commercialization of the OCT technology. In addition to exploring the feasibility of OCT in various biomedical applications, the interest in using OCT for industry non-destructive inspection has increased recently, for example, the volumetric inspection of defects present within semiconductor chips or wafers. In this talk, I will review our recent work on developing high-speed OCT technology for various biomedical applications and share preliminary results on using OCT for volumetric imaging of semiconductor wafers.

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15:45 - 16:00 Award Candidate (Paper Competition)
Manuscript ID.  0438
Paper No.  2023-FRI-S0602-O001
Yi-Chia Chen Dynamic Cell Clusters Imaging Using Full-Field Optical Coherence Tomography
Yi-Chia Chen, Bo-Chen Liu, National Taiwan University (Taiwan); Wei-Li Chen, National Taiwan University Hospital (Taiwan); Sheng-Lung Huang, National Taiwan University (Taiwan)

Full-field optical coherence tomography (FF-OCT) achieve non-invasive and high spatial resolution in cell imaging. Presently, we have developed a method to capture dynamic signals in cells using the FF-OCT system. We analyze different dynamic characteristics of cells within the frequency domain, allowing for the generation of color-coded dynamic FF-OCT image through a customized analysis program. This approach enables the exploration of various dynamic characteristics manifested by cells, thereby enhancing comprehension of the samples.

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16:00 - 16:15 Award Candidate (Paper Competition)
Manuscript ID.  0357
Paper No.  2023-FRI-S0602-O002
Yi-Chia Chen 3-D imaging of dynamic activities in human corneal epithelial cells with full-field optical coherence tomography
Yi-Chia Chen, Bo-Chen Liu, National Taiwan University (Taiwan); Wei-Li Chen, National Taiwan University Hospital (Taiwan); Sheng-Lung Huang, National Taiwan University (Taiwan)

Full-field optical coherence tomography (FF-OCT) can achieve cellular-resolution imaging non-invasively. Integrating a high-frame-rate CCD sensor and a high-brightness broadband source can pinpoint the dynamic physiological signals with micron resolution within the 3-D imaging volume at a millisecond time scale. As a demonstration, human corneal epithelial cells (HCECs) were cultivated and used as the test sample for the 3-D dynamic FF-OCT system. The dynamic behavior of the HCECs was analyzed in the frequency domain and coded by colors. The 3-D color-coded D-FF-OCT can well reveal spatial-temporal physiological cellular activities of the HCECs.

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16:15 - 16:30 Award Candidate (Paper Competition)
Manuscript ID.  0284
Paper No.  2023-FRI-S0602-O003
Zhi-Wei Xu Fluorescence Lifetime Imaging for Tissue Fibrosis Analysis
Zhi-Wei Xu, Jui-Chi Chang, Chia-Yuan Chang, National Cheng Kung University (Taiwan)

This study utilizes multiphoton excited fluorescence microscopy (MPEFM) to scan sections of fibrotic mouse lungs. By employing time-correlated single photon counting (TCSPC), fluorescence lifetime information can be directly obtained. This allows recording the fluorescence lifetime values for each pixel, which are then used to reconstruct a fluorescence lifetime image (FLIM) with spatial resolution of submicron. Finally, the phasor analysis method is applied to identify the presence of collagen type I and type III within the images.

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16:30 - 16:45 Award Candidate (Paper Competition)
Manuscript ID.  0011
Paper No.  2023-FRI-S0602-O004
Chi-Tsuan Ting Visualization of dental calculus using degree of polarization uniformity
Chi-Tsuan Ting, Chia-Wei Sun, National Yang Ming Chiao Tung University (Taiwan)

During the treatment for periodontitis, dental calculus removal is necessary. We have proposed the DAM algorithm previously for lesion identification, which enables the non-contact evaluation during the operation. However, dental calculus delineation was still flawed. Therefore, we utilized the power of polarization-sensitive optical coherence tomography and evaluated the contrast called degree of polarization uniformity for dental calculus visualization.

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16:45 - 17:00 Award Candidate (Paper Competition)
Manuscript ID.  0581
Paper No.  2023-FRI-S0602-O005
Zi-Cen Liu Assessment of active myopic choroidal neovascularization on optical computed tomography (OCT) and OCT angiography (OCTA) using deep learning algorithm
Zi-Cen Liu, Ting-Hao Chen, National Taiwan University (Taiwan); Mei-Chi Tsui, National Taiwan University, College of Medicine (Taiwan); Chen-Kuang Lee, NVIDIA (Taiwan); Tyzz-Chang Ho, National Taiwan University, College of Medicine (Taiwan); Hsiang-Chieh Lee, National Taiwan University (Taiwan)

With the rising annual prevalence of high myopia patients, the patients are often coupled with myopia choroidal neovascularization (mCNV) exhibiting the potential to induce visual impairment. This study endeavors to harness optical coherence tomography (OCT) and OCT angiography (OCTA) imaging data to build machine learning models capable of distinguishing between active and inactive mCNV cases. The ultimate objective is to curtail the necessity for frequent recourse to fluorescein angiography (FAG).

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17:00 - 17:15
Manuscript ID.  0808
Paper No.  2023-FRI-S0602-O006
Nazish Murad Incorporating a priori model information into the diffuse optical imaging of elliptical phantoms
Nazish Murad, Min Chun Pan, National Central University (Taiwan); Ya-Fen Hsu, Landseed Hospital International (Taiwan)

In order to address the limitations of circular phantoms and improve the versatility of the reconstruction approach via diffuse optical imaging (DOI), transfer learning is utilized in this work. Transfer learning is employed to adapt the previously developed architecture(s) to handle elliptical phantoms in DOI. By leveraging the knowledge and pre-trained weights obtained from the circular phantom dataset, the network is fine-tuned using the newly acquired elliptical phantom dataset. This approach can potentially enhance the realism and accuracy of DOT imaging, enabling more precise characterization of biological tissues and structures.

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17:15 - 17:30
Manuscript ID.  0905
Paper No.  2023-FRI-S0602-O007
Dr. AJEETKUMAR PATIL Design and Development of Biophotonics Tools for Biomedical Applications.

Biophotonics tools offer noninvasive and easily applicable tools for the detection of alternations in structural and biochemical compositions of tissues and cells, which may indicate the presence of disease. The present work discusses about developing reliable, sensitive and objective biophotonics tools for various biomedical applications. Our methods have the added advantage of being label-free, objective because of the fact that diagnostic evaluation is by statistical methods, eliminating errors from lack of experience, fatigue factor, and subjectivity in the decision making.

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